KEY FEATURES REQUIRED FOR A DNA POLYMERASE FOR POLYMERASE CHAIN REACTION
DNA polymerase is a key enzyme used in the polymerase chain reaction (PCR) process, which is essential for amplifying specific DNA sequences. The key features required for a DNA polymerase to be suitable for PCR include: Thermal Stability: The DNA…
OVERVIEW OF THE POLYMERASE CHAIN REACTION METHODOLOGY
The Polymerase Chain Reaction (PCR) is a powerful and widely used technique in molecular biology for amplifying specific DNA sequences. Developed by Kary Mullis in 1983, PCR has become an indispensable tool in various fields, including genomics, diagnostics, and forensics.…
ACTIVATION OF AMINO ACIDS AND ELONGATION OF THE POLYPEPTIDE CHAIN IN PROKARYOTES
Prokaryotes, such as bacteria, have a unique process of protein synthesis compared to eukaryotes, such as humans. The process of protein synthesis in prokaryotes involves the activation of amino acids, the formation of an initiation complex, and the elongation of…
DESIGNING GENE-SPECIFIC CHAIN REACTION PRIMERS AND AMPLIFICATION OF DNA
PCR (Polymerase Chain Reaction) is a powerful molecular biology technique used to amplify specific DNA sequences. The process involves the use of primers, which are short DNA sequences that bind to the target DNA sequence and initiate the PCR reaction.…
BELT AND CHAIN DRIVES
A belt drive system is a method of transferring rotary motion between two shafts. A belt drive includes one pulley on each shaft and one or more continuous belts over the two pulleys. The motion of the driving pulley is…