A COMPREHENSIVE OVERVIEW OF BLUNT END RESTRICTION ENZYMES
Blunt end restriction enzymes, also known as blunt cutters or blunt-end cutters, are a type of restriction enzyme that cleave DNA molecules in a manner that results in blunt ends. These enzymes recognize specific DNA sequences and cut the DNA…
BLUNT END RESTRICTION ENZYMES AND VECTOR DIGESTION
In molecular biology, a vector is a DNA molecule that carries a specific gene of interest. In this case, the vector carries a blunt end restriction enzyme releasing a β-galactosidase gene and a phosphatase gene. Restriction enzymes are nucleases that…
TYPES OF REPLICATION ENZYMES IN EUKARYOTES
Eukaryotic cells possess a complex and highly regulated process of DNA replication, which is essential for the accurate transmission of genetic information during cell division. The replication of eukaryotic DNA is carried out by a set of enzymes that work…
TYPES OF REPLICATION ENZYMES IN PROKARYOTES
Prokaryotic cells, which include bacteria and archaea, possess a simpler structure compared to eukaryotic cells. One of the essential processes in prokaryotic cells is DNA replication, which is carried out by a set of enzymes. DNA replication is the process…
CELLULAR ACTION OF GLYCOSYLTRANSFERASE ENZYMES FOR O-LINKED AND N-LINKED GLYCAN BIOSYNTHESIS
Glycosyltransferases are enzymes responsible for the biosynthesis of glycoproteins, which are proteins that have sugar molecules attached to them. These enzymes play a crucial role in the addition of sugar moieties to proteins, thereby influencing various cellular processes such as…
INTERPRETATION OF CARDIAC ENZYMES
Introduction Cardiac enzymes, also known as cardiac biomarkers, are proteins released into the blood when the heart muscle is damaged due to various conditions such as myocardial infarction (heart attack), cardiomyopathy, or other forms of heart disease. The levels of…
EXPERIMENTS TO SHOW THE CHARACTERISTICS OF ENZYMES
Experiment 1 Aim: To demostrate the action of ptyalin (saliva) on cooked starch. Method: Some saliva is introduced into a test tube X and diluted with little amount of water. Two test tubes are labelled E and F. To test…
DIGESTIVE ENZYMES
Enzymes are organic catalysts of protein origin produced by living cells which help to speed up or slow down the rate of chemical reactions but remain chemically unchanged at the end of the reaction. Classes of enzymes Enzymes are…