INTRODUCTION TO NUCLEASE PROTECTION ASSAY
The Nuclease Protection Assay, also known as the RNase Protection Assay or Ribonuclease Protection Assay, is a highly sensitive and specific technique used to detect, quantify, and analyze the structure and stability of RNA molecules. This method was developed in…
YEAST TWO-HYBRID ASSAY EXPLAINED
Introduction The yeast two-hybrid (Y2H) assay is a powerful molecular biology technique used to detect protein-protein interactions in vivo. This assay is based on the reconstitution of a transcription factor through the interaction of two proteins of interest, leading to…
PERFORMING THE THERMOFLUOR ASSAY WITH AND WITHOUT BOUND DRUGS
Performing and interpreting biophysical analyses of a protein with and without bound drugs using the Thermofluor assay involves several steps. The Thermofluor assay, also known as thermal shift assay or differential scanning fluorimetry, is a powerful tool for studying protein…
A GUIDE TO GST PULL-DOWN-BASED INTERACTION ASSAY
The GST pull-down-based interaction assay is a widely used technique to study protein-protein interactions. This assay involves the use of glutathione S-transferase (GST) fusion proteins to pull down interacting proteins from a cell lysate or in vitro translation system. The…
GENERAL STEPS INVOLVED IN DEVELOPING PROTEIN ASSAY PROTOCOLS
Protein assays are laboratory techniques used to measure the presence, concentration, or activity of proteins in a sample. These assays are crucial in various fields, including biomedical research, drug discovery, and diagnostics. Developing assay protocols is an essential step in…
Study Guide
