A COMPREHENSIVE OVERVIEW OF BLUNT END RESTRICTION ENZYMES
Blunt end restriction enzymes, also known as blunt cutters or blunt-end cutters, are a type of restriction enzyme that cleave DNA molecules in a manner that results in blunt ends. These enzymes recognize specific DNA sequences and cut the DNA…
BLUNT END RESTRICTION ENZYMES AND VECTOR DIGESTION
In molecular biology, a vector is a DNA molecule that carries a specific gene of interest. In this case, the vector carries a blunt end restriction enzyme releasing a β-galactosidase gene and a phosphatase gene. Restriction enzymes are nucleases that…