PRIMER DESIGN FOR PCR METHODS
Primer design is a critical aspect of PCR (polymerase chain reaction) methods, as it directly impacts the success and specificity of the amplification process. Whether for restriction-ligation or Gibson Assembly, the design of primers requires careful consideration of various factors…
BENCHLING FOR MOLECULAR CLONING WORKFLOW DESIGN AND RECORD KEEPING
Benchling is a powerful platform that offers solutions for molecular cloning workflow design and record keeping. It provides a comprehensive suite of tools to streamline the entire process, from experimental design to data analysis and collaboration. Here’s a detailed overview…
DESIGN AND ASSEMBLY OF RECOMBINANT PLASMID CONSTRUCTS FOR PROTEIN EXPRESSION
In order to design and assemble ad hoc combinations of DNA precursors in silico and then in the laboratory to build recombinant plasmid constructs capable of directing expression of a recombinant protein, several steps and considerations need to be taken…
MECHANISMS OF INHIBITION AND APPLICATIONS TO STRUCTURE-BASED DRUG DESIGN AND DRUG DISCOVERY
In the field of pharmaceutical chemical biology, understanding the mechanisms of inhibition is crucial for the development of effective drugs. Inhibition refers to the process by which a molecule, typically a drug or inhibitor, binds to a target molecule, such…
Study Guide
