HOW THE PLASMID CAN BE USED TO GENETICALLY MODIFY A BACTERIAL CELL TO CONTAIN A HUMAN GENE
Introduction to Plasmids Plasmids are small, circular DNA molecules that are separate from the chromosomal DNA and are found in bacteria and other organisms. They play a crucial role in genetic engineering as they can be used as vectors to…
CLONING cDNA IN PLASMID VECTORS
Cloning cDNA in plasmid vectors is a fundamental technique in molecular biology that allows for the replication and expression of specific DNA sequences. This process involves the insertion of complementary DNA (cDNA) into a plasmid vector, which can then be…
DESIGN AND ASSEMBLY OF RECOMBINANT PLASMID CONSTRUCTS FOR PROTEIN EXPRESSION
In order to design and assemble ad hoc combinations of DNA precursors in silico and then in the laboratory to build recombinant plasmid constructs capable of directing expression of a recombinant protein, several steps and considerations need to be taken…
PLASMID TRANSFORMATION INTO ESCHERICHIA COLI
Plasmid transformation into Escherichia coli is a widely used technique in molecular biology to introduce foreign DNA into bacterial cells. This process is essential for various applications such as cloning, protein expression, and genetic engineering. The transformation process involves the…
Study Guide
