INTRODUCTION TO GENETIC CIRCUITS AND RECOMBINANT PROTEIN EXPRESSION
Genetic circuits are a set of interconnected genes and regulatory elements that work together to control cellular processes. In the context of optimizing recombinant protein expression, genetic circuits can be designed to enhance the production of desired proteins within host…
DESIGN AND ASSEMBLY OF RECOMBINANT PLASMID CONSTRUCTS FOR PROTEIN EXPRESSION
In order to design and assemble ad hoc combinations of DNA precursors in silico and then in the laboratory to build recombinant plasmid constructs capable of directing expression of a recombinant protein, several steps and considerations need to be taken…
THE T7 RECOMBINANT EXPRESSION SYSTEM
The T7 recombinant expression system is a widely used method for the production of recombinant proteins in bacteria. This system utilizes the T7 RNA polymerase to drive the expression of target genes cloned downstream of a T7 promoter. The T7…
RECOMBINANT DNA TECHNOLOGY
Recombinant DNA technology, also known as genetic engineering, refers to a set of molecular techniques used to manipulate the genetic material of an organism, typically DNA. The process involves cutting and splicing together pieces of DNA from different sources, such…
Study Guide
