OVERVIEW OF CLONING VECTORS, PROMOTERS AND EXPRESSION VECTOR
a) Cloning Vectors Cloning vectors are DNA molecules used to “carry” foreign DNA into a host organism, where it can be replicated and manipulated. There are several types of cloning vectors, each with its own unique features and applications. The…
CLONING LARGE DNA FRAGMENTS IN BAC AND YAC VECTORS
Bacterial Artificial Chromosome (BAC) and Yeast Artificial Chromosome (YAC) vectors are widely used in molecular biology for cloning large DNA fragments. These vectors are essential tools for studying complex genomes, constructing genomic libraries, and manipulating large DNA fragments. Cloning large…
Ti PLASMIDS AS VECTORS FOR PLANT CELLS
Ti plasmids, also known as tumor-inducing plasmids, are a group of circular DNA molecules found in Agrobacterium tumefaciens, a soil bacterium. These plasmids have been extensively studied and utilized as vectors for introducing foreign DNA into plant cells. The ability…
CLONING cDNA IN PLASMID VECTORS
Cloning cDNA in plasmid vectors is a fundamental technique in molecular biology that allows for the replication and expression of specific DNA sequences. This process involves the insertion of complementary DNA (cDNA) into a plasmid vector, which can then be…
BACTERIA AS VECTORS FOR AMPLIFYING FOREIGN DNA
Genetic engineering involves the manipulation of an organism’s genetic material using biotechnology. One common method in genetic engineering is the use of bacteria as vectors for amplifying foreign DNA. This process has revolutionized various fields such as medicine, agriculture, and…
Study Guide
