BACTERIAL ARTIFICIAL CHROMOSOMES IN FOCUS
Bacterial artificial chromosomes (BACs) are vectors used to clone DNA fragments in bacteria. They are widely used in molecular biology for various applications, including genomic sequencing, functional genomics, and transgenic animal production. BACs are particularly valuable for their ability to…
METHODS TO DETERMINE HOST BACTERIAL CELLS CONTAINING CLONAL INSERT
1. Selection Markers One method to determine which host bacterial cells contain the clonal insert is by using selection markers. These markers are genes that confer a selective advantage to the host cell when they are present. For example, if…
HOW THE PLASMID CAN BE USED TO GENETICALLY MODIFY A BACTERIAL CELL TO CONTAIN A HUMAN GENE
Introduction to Plasmids Plasmids are small, circular DNA molecules that are separate from the chromosomal DNA and are found in bacteria and other organisms. They play a crucial role in genetic engineering as they can be used as vectors to…
UNLOCKING THE SECRETS OF BACTERIAL TRANSPOSABLE ELEMENTS
Bacterial transposable elements, also known as transposons, are DNA sequences that have the ability to move from one location to another within a bacterial genome. They play a significant role in bacterial genetics and evolution, contributing to genetic diversity and…
BACTERIAL REPLICATION, STRUCTURE, ADHESION, MOTILITY AND GROWTH
Bacteria are single-celled microorganisms that can be found in various environments. They are known for their ability to replicate, their diverse structures, adhesion capabilities, motility, and growth patterns. Bacterial Replication Bacterial replication is the process by which bacteria reproduce. It…
AN OVERVIEW OF BACTERIAL TRANSFORMATION BY PLASMIDS
Bacterial transformation is a process in which bacteria take up foreign genetic material, such as plasmids, from the surrounding environment. This natural process has been widely utilized in genetic engineering and biotechnology to introduce new genes into bacterial cells. Plasmids…
RESTRICTION MAPPING OF PGLO AND TRANSFORMATION OF BACTERIAL CELLS
The pGLO plasmid is a common tool in molecular biology used to study gene expression and protein localization. Restriction mapping of the pGLO plasmid involves the use of restriction enzymes to cleave the DNA at specific recognition sites, allowing for…
A CLOSER LOOK AT BACTERIAL SEPSIS IN NEWBORNS
Bacterial sepsis in newborns, also known as neonatal sepsis, is a serious medical condition characterized by a systemic infection in the first month of life. It is a leading cause of morbidity and mortality in newborns, particularly those born prematurely…
STRUCTURE OF BACTERIAL CELL AND SPECIALIZED STRUCTURES
A typical bacterial cell is a single-celled microorganism that belongs to the domain Bacteria. It is characterized by its simplicity and lack of a true nucleus or membrane-bound organelles. Despite its simplicity, a bacterial cell contains several important components that…
UNLOCKING THE SECRETS OF BACTERIAL GENETICS
Introduction Bacterial genetics is the study of how genetic information is passed on within bacterial populations. Bacteria have a single circular chromosome and can also contain plasmids, which are small, independent pieces of DNA. They reproduce primarily through binary fission,…
BACTERIAL INFECTIONS OF MEDICAL IMPORTANCE
Nationally notifiable bacterial diseases For the most up-to-date information, I recommend referring to the Centers for Disease Control and Prevention (CDC) website or other official health agencies. Anthrax Botulism (foodborne, wound, and infant) Brucellosis Chancroid Chlamydia trachomatis infection Cholera Diphtheria…
BACTERIAL IDENTIFICATION PROCEDURES
Specimen Collection Guidelines Proper collection of specimens is crucial for accurate diagnosis and effective treatment of various medical conditions. Here are some guidelines for collecting different types of specimens: Throat Swab: Wash your hands and put on gloves. Ask the…
HOW TO IDENTIFY AND TREAT BACTERIAL INFECTIONS OF THE SKIN
Skin commensals and pathogens Cultural Characteristics of Skin Commensals: Diversity: The human skin harbors a diverse array of commensal microorganisms, including bacteria, fungi, viruses, and mites. These organisms are part of the skin’s natural microbiota and vary in their species…
Study Guide
