OVERVIEW OF CLONING VECTORS, PROMOTERS AND EXPRESSION VECTOR
a) Cloning Vectors Cloning vectors are DNA molecules used to “carry” foreign DNA into a host organism, where it can be replicated and manipulated. There are several types of cloning vectors, each with its own unique features and applications. The…
CLONING LARGE DNA FRAGMENTS IN BAC AND YAC VECTORS
Bacterial Artificial Chromosome (BAC) and Yeast Artificial Chromosome (YAC) vectors are widely used in molecular biology for cloning large DNA fragments. These vectors are essential tools for studying complex genomes, constructing genomic libraries, and manipulating large DNA fragments. Cloning large…
EXPLORING THE CLONING PROCESS OF ‘DOLLY’ THE SHEEP
Cloning is the process of creating a genetically identical copy of an organism. In the case of ‘Dolly’ the sheep, she was the first mammal to be cloned from an adult somatic cell using a technique called somatic cell nuclear…
CLONING cDNA IN PLASMID VECTORS
Cloning cDNA in plasmid vectors is a fundamental technique in molecular biology that allows for the replication and expression of specific DNA sequences. This process involves the insertion of complementary DNA (cDNA) into a plasmid vector, which can then be…
THE PROCESS OF CLONING AN ORGANISM FROM MRNA
Cloning an organism from mRNA is a complex process that involves several steps. Here’s a general overview of the process: 1) mRNA isolation The first step is to isolate the mRNA from the cell or tissue of interest. This can…
BIOINFORMATICS FOR MOLECULAR CLONING
Bioinformatics plays a crucial role in molecular cloning, which is the process of creating identical copies of a DNA sequence. It involves the use of computational tools and databases to analyze and manipulate biological data, aiding in the design and…
BENCHLING FOR MOLECULAR CLONING WORKFLOW DESIGN AND RECORD KEEPING
Benchling is a powerful platform that offers solutions for molecular cloning workflow design and record keeping. It provides a comprehensive suite of tools to streamline the entire process, from experimental design to data analysis and collaboration. Here’s a detailed overview…
DESIGNING A CLONING EXPERIMENT
Cloning is the process of creating a genetically identical copy of an organism. In the context of molecular biology, cloning often refers to the process of making multiple copies of a specific DNA sequence. Here’s a comprehensive plan for designing…
Study Guide
