GENERAL KNOWLEDGE

EXPERIMENTAL VERIFICATION OF LAMBERT-BEER’S LAW

Lambert-Beer’s Law, also known as Beer’s Law, is a fundamental concept in the field of spectrophotometry and absorption spectroscopy. It describes the relationship between the absorbance of a solution, its concentration, the path length, and the molar absorptivity of the absorbing species. The law states that the absorbance of a solution is directly proportional to the concentration of the absorbing species, the path length of the light through the solution, and the molar absorptivity of the species.

To verify Lambert-Beer’s Law, experiments are conducted using different concentrations of the absorbing species, varying path lengths, and constant molar absorptivity. The absorbance is measured for each condition, and a plot of absorbance versus concentration is created. If the data points follow a linear relationship, it confirms the validity of the law.

  1. Beer’s Law Experiment with Different Concentrations: In this experiment, a constant path length is chosen, and the absorbance is measured for different concentrations of the absorbing species. The data is plotted, and if a straight line is obtained, it confirms the validity of the law.
  2. Beer’s Law Experiment with Different Path Lengths: In this experiment, a constant concentration of the absorbing species is chosen, and the absorbance is measured for different path lengths of the light through the solution. The data is plotted, and if a straight line is obtained, it confirms the validity of the law.
  3. Combined Beer’s Law Experiment: In this experiment, a series of experiments are conducted using different concentrations and path lengths. The data obtained from these experiments is combined, and a plot of absorbance versus concentration is created. If a straight line is obtained, it confirms the validity of the law.
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